Shakable Liquid Culture Jar Kit

$29.95

Make or expand your own liquid cultures by 500ml with this kit. No-Stir plate, stir rod or marble needed.
1 Quart Ball Mason Jar. Lid with hydrophobic 0.22-micron filter, mycelium shredder and silicon injection port. Contains 5 x 5ml Syringes and 5 x 18 gauge needles to get you started.
Contains Liquid Culture Media powder for making 500ml of media. Contains light malt or dextrose, peptone and bakers yeast.

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SKU: lcjarlidmediapwdr Category:

Description

Make or expand your own liquid cultures with this kit. No-Stir plate, stir rod or marble needed.
Contains 5 x 5ml Syringes and 5 x 18 gauge needles to get you started.
This liquid culture kit has a lid with a special 0.22-micron filter that repels the liquid media and only allowing air exchange.
This allows the jar to be shaken without contaminating the filter media stopping bacteria and mold growth in the filter.
An 18g needle is attached to the filter to help shred the mycelium into smaller particles when shaken allowing faster colonisation and easier drawing of the media into a syringe. It also adds more protection from the liquid media coming into contact with the filter.

The silicon port is self-healing and allows the injection of a liquid culture or contaminate free spore syringe.
When incubated at the right temperature the liquid culture in the jar can be ready for use in 2 – 4 weeks (depending on species).

To prepare the Liquid culture you will need a pressure cooker that can reach 15psi.

  1. Remove the lid,
  2. Add the liquid culture ingredients to the jar,
  3. Add 500ml of warm water (no more than 500ml),
  4. Carefully remove the needle protector from the needle on the underside of the lid,
  5. Screw the lid on tightly,
  6. Shake the contents of the jar with one finger covering the filter hole enough to dissolve the Dextrose/Light malt (the yeast and may not dissolve),
  7. Add spacers to the bottom of a pressure cooker for the jar to sit on out of the water,
  8. Add enough water to run the pressure cooker for 30minutes,
  9. Run the pressure cooker on high pressure (15psi). Bring it up to pressure and then cook for 25 minutes.
  10. LET THE PRESSURE COOKER COOL WITHOUT MANUALLY SUDDENLY RELEASING PRESSURE.

To introduce Culture/spores via the injection port.

  1. Let the jar cool to room temperature,
  2. Wipe the injection port with an alcohol wipe or isopropyl alcohol spray (70%+ alcohol),
  3. Flame the Culture/Spore Needle until it glows red. Do  not use if there is soot on the needle,
  4. Let the needle cool for 10 seconds then push it through the injection port. No too far that it hit the end of the needle,, this will damage the port,
  5. Inject the contents of the syringe (usually 5 – 10ml).
  6. with fingers on either side of the needle, pushing down on the port, withdraw the needle.
  7. Wipe or spray the injection port again with alcohol.

To draw out the liquid culture.

  1. With a clean dry but not sterile syringe gently push 5ml of air through the filter (releases any blockages that may be present in the filter needle). DO NOT DRAW,
  2. Wipe the injection port with an alcohol wipe or isopropyl alcohol spray (70%+ alcohol),
  3. Attach a new sterile needle to a new sterile syringe.
  4. Flame the Needle until it glows red. Do  not use if there is soot on the needle,
  5. Let the needle cool for 10 seconds then push it through the injection port. No too far that it hit the end of the needle, this will damage the port,
  6. Tilt the jar so that the injection port is submerged in the culture but never the filter needle. The port should be at the bottom and the filter should be at the top when the jar is laid on its side,
  7. Draw the required amount into the syringe. If it gets blocked push the contents of the string back into the jar and try drawing again,
  8. With fingers on either side of the needle, pushing down on the port, withdraw the needle.
  9. Wipe or spray the injection port again with alcohol.

Shake the jar vigorously once a week to prevent the mycelium from forming clumps and mats.

A liquid culture powder is also available and contains special ingredients to support healthy long-lasting mycelium.
Liquid Culture Ingredients:
Light Malt or Dextrose
Laboratory grade bacteriological peptone
Bakers Yeast
It is advised that the addition of 3 drops of vegetable oil and a toothpick can be added to prolong the life of the culture.

Additional information

Weight N/A
Dimensions N/A
Media Type

Light Malt, Dextrose

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